资源类型

期刊论文 49

年份

2023 3

2022 5

2021 6

2020 1

2019 6

2018 3

2017 2

2016 4

2015 1

2014 4

2013 2

2012 4

2010 1

2009 2

2008 1

2007 1

2006 1

2001 1

展开 ︾

关键词

ABC-F家族蛋白 1

MERS-CoV 1

MS-CETSA 1

SrpA 1

“白箱”模型 1

一次原因分析法 1

中国航天白皮书 1

人工智能诊断 1

人造水通道 1

仿生膜 1

刺突蛋白 1

多孔碳 1

大航天 1

实时监测网络 1

微波吸收 1

抗疟 1

核糖体 1

氧化铝 1

水通道蛋白 1

展开 ︾

检索范围：

排序： 展示方式：

reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 373-381 doi: 10.15302/J-FASE-2018210

摘要：

Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.

关键词： Aspergillus oryzae     egg allergy     egg white proteins     IgE-binding ability     ovomucoid    

HTML PDF 收藏

EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

Ning YANG

《农业科学与工程前沿（英文）》 2021年 第8卷 第1期   页码 25-34 doi: 10.15302/J-FASE-2020363

摘要： Eggs are one of the most nutritious and affordable animal products worldwide. From 1985, egg production in China has retained the leading place in the world. A total of 33 Mt of eggs were produced in 2019 representing ˃ 40% of the world total production. Egg production in China is characterized by diversity in several aspects, including layer breeds, products and production systems. New breeds and synthetic lines are developed to improve the genetic potentials of egg production and feed efficiency of layers. In the past, layer farms were run mostly by small households with 100 to 1000 layers per farm. Over the past decades, egg production in China has developed toward standardization and expansion of production systems, and many of these modern intensive farms raise millions of layers. Although the Chinese egg products maintain strong competitiveness over other animal products and imported egg products, the egg industry will grow at a slower pace compared to the past. Chinese consumers are more concerned about the quality and safety of eggs and egg products, as well as the environmental issues related to animal production, which presents challenges for the Chinese egg industry.

关键词： poultry farms     China     diversity     egg production     trends    

HTML PDF 收藏

Novel engineered proteins for mechanomaterials

Giuseppe Portale

《化学科学与工程前沿（英文）》 2020年 第14卷 第6期   页码 1122-1123 doi: 10.1007/s11705-020-1941-x

HTML PDF 收藏

Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

《医学前沿（英文）》 2021年 第15卷 第6期   页码 887-902 doi: 10.1007/s11684-021-0879-9

摘要： Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2+SOX10+ oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.

关键词： microRNAs     monocarboxylate transporter 1     motor training     subarachnoid hemorrhage     white matter injury    

HTML PDF 收藏

Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 216-223 doi: 10.1007/s11705-012-1289-y

摘要： White spot syndrome virus (WSSV) is a major cause of high mortality in cultured shrimp all over the world. VP26 is one of the structural proteins of WSSV that is assumed to assist in recognizing its host and assists the viral nucleocapsid to move toward the nucleus of the host cell. The objective of this work was to produce a polyclonal antibody against VP26 and use it as a biosensor. The recombinant VP26 protein (rVP26) was produced in (BL21), purified and used for immunizing rabbits to obtain a polyclonal antibody. Western blot analysis confirmed that the antiserum had a specific immunoreactivity to the VP26 of WSSV. This VP26 antiserum was immobilized onto a gold electrode for use as the sensing surface to detect WSSV under a flow injection system. The impedance change in the presence of VP26 was monitored in real time. The sensitivity of the biosensor was in the linear range of 160–160000 copies of WSSV, indicating that it is good and sensitive for analysis of WSSV. The specificity of the biosensor was supported by the observation that no impedance change was detected even at high concentrations when using Yellow Head Virus (YHV). This biosensor may be applied to monitor the amount of WSSV in water during shrimp cultivation.

关键词： recombinant protein     polyclonal antibody     label-free biosensor     impedance     white spot syndrome virus (WSSV)    

HTML PDF 收藏

源于蛋清的新型分层轻质多孔碳用于高效微波吸收 Article

刘宗林, 赵旭, 徐亮亮, 彭庆宇, 赫晓东

《工程（英文）》 2022年 第18卷 第11期   页码 161-172 doi: 10.1016/j.eng.2022.04.026

摘要：

鸡蛋羹是一种在餐桌上常见的菜肴，在冷冻干燥后可得到均匀的多孔结构。鸡蛋羹中的蛋白质提供了丰富的碳和氮元素，并且鸡蛋羹的独特微观结构和可调节的电学参数使它成为一种潜在的多孔碳前驱体。本文以蛋清作为原材料，氮原位掺杂的多孔碳（NPC）和碳酸钾改性的NPC（PNPC）是通过一个简单的凝胶和碳化过程制备得到的。多孔碳的独特形貌继承于蛋白质，包括纤维簇、蜂窝孔和布满沟槽的骨架。这些结构具有优异的阻抗匹配和高效的内部损耗性能，使得到的多孔碳成为优异的无需金属元素掺杂的轻质电磁波吸收材料。作为多孔碳的代表之一，PNPC10-700 具有包括纤维簇、蜂窝孔和多孔骨架的多重
结构。并且，PNPC10-700 具有最大反射损耗值（66.15 dB；厚度为3.77 mm）和一个宽达5.82 GHz的有效吸收频段（从12.18 GHz到18 GHz，厚度为2.5 mm），这远超大部分文献中报道的数值。因此蛋清（蛋白质）的凝胶和后续碳化的结合是一种用于设计多孔碳吸波材料微观形貌和电磁性能的新方法。

关键词： 微波吸收     多孔碳     生物质     碳酸钾    

HTML PDF 收藏

EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

《农业科学与工程前沿（英文）》 2021年 第8卷 第1期   页码 25-34

摘要：

• China is now the largest egg production country worldwide

• Egg production in China is characterized by diversity in several aspects

• China is now capable of breeding new varieties, with more than 50% of the market share

• Policies have been implemented to ensure sustainable development of egg production

• Integrating crop-chicken-vegetable production system is established

 

Eggs are one of the most nutritious and affordable animal products worldwide. From 1985, egg production in China has retained the leading place in the world. A total of 33 Mt of eggs were produced in 2019 representing ˃ 40% of the world total production. Egg production in China is characterized by diversity in several aspects, including layer breeds, products and production systems. New breeds and synthetic lines are developed to improve the genetic potentials of egg production and feed efficiency of layers. In the past, layer farms were run mostly by small households with 100 to 1000 layers per farm. Over the past decades, egg production in China has developed toward standardization and expansion of production systems, and many of these modern intensive farms raise millions of layers. Although the Chinese egg products maintain strong competitiveness over other animal products and imported egg products, the egg industry will grow at a slower pace compared to the past. Chinese consumers are more concerned about the quality and safety of eggs and egg products, as well as the environmental issues related to animal production, which presents challenges for the Chinese egg industry.

HTML PDF 收藏

Paradoxical role of Id proteins in regulating tumorigenic potential of lymphoid cells

null

《医学前沿（英文）》 2018年 第12卷 第4期   页码 374-386 doi: 10.1007/s11684-018-0652-x

摘要：

A family of transcription factors known as Id proteins, or inhibitor of DNA binding and differentiation, is capable of regulating cell proliferation, survival and differentiation, and is often upregulated in multiple types of tumors. Due to their ability to promote self-renewal, Id proteins have been considered as oncogenes, and potential therapeutic targets in cancer models. On the contrary, certain Id proteins are reported to act as tumor suppressors in the development of Burkitt’s lymphoma in humans, and hepatosplenic and innate-like T cell lymphomas in mice. The contexts and mechanisms by which Id proteins can serve in such contradictory roles to determine tumor outcomes are still not well understood. In this review, we explore the roles of Id proteins in lymphocyte development and tumorigenesis, particularly with respect to inhibition of their canonical DNA binding partners known as E proteins. Transcriptional regulation by E proteins, and their antagonism by Id proteins, act as gatekeepers to ensure appropriate lymphocyte development at key checkpoints. We re-examine the derailment of these regulatory mechanisms in lymphocytes that facilitate tumor development. These mechanistic insights can allow better appreciation of the context-dependent roles of Id proteins in cancers and improve considerations for therapy.

关键词： Id proteins     lymphoma     leukemia     T cells     B cells     tumor suppressor     oncogene    

HTML PDF 收藏

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 299-306 doi: 10.15302/J-FASE-2014040

摘要： Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes. Unlike the melanocytes that are confined to the integument in other vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration. In this study, we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl. Differential expression of 2517 microarray probes ( <0.01, Fold Change>2) was observed in Silky Fowl compared to White Leghorn. After filtration by cluster analysis, functional annotation and pathway analysis, eight differentially expressed genes were identified to be closely related to the development of melanocytes. Moreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn. The differentially expressed genes associated with melanocyte development were verified by q-PCR, and results were highly consistent with the microarray data. The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl, although there were no big differences in melanoblast development between these two breeds. The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.

关键词： Silky Fowl     White Leghorn     melanoblast migration     gene expression    

HTML PDF 收藏

Caveolin proteins: a molecular insight into disease

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 397-404 doi: 10.1007/s11684-016-0483-6

摘要：

Caveolae are a kind of specific cystic structures of lipid rafts in the cytoplasmic membrane and are rich in cholesterol and sphingolipids. In recent years, many researchers have found that both caveolins and caveolae play a role in the development of various human diseases, including coronary heart disease, hypertension, and nervous system disorders. The specific mechanisms by which caveolins induce diseases have been a topic of interest. However, a number of detailed molecular mechanisms remain poorly understood. This article focuses on the relationship between caveolin proteins and human diseases and reviews the molecular mechanisms of caveolins in disease networks.

关键词： caveolin     caveolae     microRNA     disease     signaling pathway     heart     tumor    

HTML PDF 收藏

control of structural responses using an optimal passive tuned mass damper under stationary Gaussian white

Min-Ho CHEY, Jae-Ung KIM

《结构与土木工程前沿（英文）》 2012年 第6卷 第3期   页码 267-280 doi: 10.1007/s11709-012-0170-x

摘要： In this study, the structural control strategy utilizing a passive tuned mass damper (TMD) system as a seismic damping device is outlined, highlighting the parametric optimization approach for displacement and acceleration control. The theory of stationary random processes and complex frequency response functions are explained and adopted. For the vibration control of an undamped structure, the optimal parameters of a TMD, such as the optimal tuning frequency and optimal damping ratio, to stationary Gaussian white noise acceleration are investigated by using a parametric optimization procedure. For damped structures, a numerical searching technique is used to obtain the optimal parameters of the TMD, and then the explicit formulae for these optimal parameters are derived through a sequence of curve-fitting schemes. Using these specified optimal parameters, several different controlled responses are examined, and then the displacement and acceleration based control effectiveness indices of the TMD are examined from the view point of RMS values. From the viewpoint of the RMS values of displacement and acceleration, the optimal TMDs adopted in this study shows clear performance improvements for the simplified model examined, and this means that the effective optimization of the TMD has a good potential as a customized target response-based structural strategy.

关键词： tuned mass damper     parametric optimization     passive control     white noise     earthquake excitation    

HTML PDF 收藏

Changes in structure and functional properties of whey proteins induced by high hydrostatic pressure:

Xiaoming LIU, Jia NING, Stephanie CLARK,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 436-442 doi: 10.1007/s11705-009-0251-0

摘要： High hydrostatic pressure (HHP) is an alternative technology to heat processing for food product modifications. It does not cause environmental pollution and eliminates the use of chemical additives in food products. This review covers the research conducted to understand the effect of HHP on structure and functional properties of whey proteins. In this paper, the mechanism underlying pressure-induced changes in -lactoglobulin and -lactabumin is also discussed and how they related to functional properties such as hydrophobicity, foam stability, and flavor-binding capacity.

关键词： -lactoglobulin     processing     functional     alternative technology     environmental    

HTML PDF 收藏

Cross-sectional network analysis of plasma proteins/metabolites correlated with pathogenesis and therapeutic

《医学前沿（英文）》 doi: 10.1007/s11684-023-1022-x

摘要： The treatment of PML/RARA+ acute promyelocytic leukemia (APL) with all-trans-retinoic acid and arsenic trioxide (ATRA/ATO) has been recognized as a model for translational medicine research. Though an altered microenvironment is a general cancer hallmark, how APL blasts shape their plasma composition is poorly understood. Here, we reported a cross-sectional correlation network to interpret multilayered datasets on clinical parameters, proteomes, and metabolomes of paired plasma samples from patients with APL before or after ATRA/ATO induction therapy. Our study revealed the two prominent features of the APL plasma, suggesting a possible involvement of APL blasts in modulating plasma composition. One was characterized by altered secretory protein and metabolite profiles correlating with heightened proliferation and energy consumption in APL blasts, and the other featured APL plasma-enriched proteins or enzymes catalyzing plasma-altered metabolites that were potential trans-regulatory targets of PML/RARA. Furthermore, results indicated heightened interferon-gamma signaling characterizing a tumor-suppressing function of the immune system at the first hematological complete remission stage, which likely resulted from therapy-induced cell death or senescence and ensuing supraphysiological levels of intracellular proteins. Overall, our work sheds new light on the pathophysiology and treatment of APL and provides an information-rich reference data cohort for the exploratory and translational study of leukemia microenvironment.

关键词： acute promyelocytic leukemia     plasma proteomics     plasma metabolomics     cross-sectional correlation network     pathogenesis     treatment    

HTML PDF 收藏

Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 362-372 doi: 10.15302/J-FASE-2018206

摘要：

This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.

关键词： Hippo signaling pathway     meat protein     PEPT1     proteome analysis     rat jejunum    

HTML PDF 收藏

Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis

Hui Wan,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhou

《化学科学与工程前沿（英文）》 2017年 第11卷 第1期   页码 72-88 doi: 10.1007/s11705-016-1580-4

摘要： Pyrroloquinoline quinone (PQQ) plays a significant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms remain unclear. Herein, we used both two-dimensional fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects of overexpression in an industrial strain of WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that , and were upregulated in all conditions. Then the three genes were over-expressed in WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 (a transporter) and B932_2186 (an ABC transporter permease) overexpression strains were enhanced by 1.77-fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932_2186 might enhance the PQQ secretion process.

关键词： 2D-DIGE     pqqB     pyrroloquinoline quinone     RNA-Seq     Vitamin C    

HTML PDF 收藏

标题 作者 时间 类型 操作

reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

期刊论文

EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

Ning YANG

期刊论文

Novel engineered proteins for mechanomaterials

Giuseppe Portale

期刊论文

Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

期刊论文

Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

期刊论文

源于蛋清的新型分层轻质多孔碳用于高效微波吸收

刘宗林, 赵旭, 徐亮亮, 彭庆宇, 赫晓东

期刊论文

EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

期刊论文

Paradoxical role of Id proteins in regulating tumorigenic potential of lymphoid cells

null

期刊论文

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

期刊论文

Caveolin proteins: a molecular insight into disease

null

期刊论文

control of structural responses using an optimal passive tuned mass damper under stationary Gaussian white

Min-Ho CHEY, Jae-Ung KIM

期刊论文

Changes in structure and functional properties of whey proteins induced by high hydrostatic pressure:

Xiaoming LIU, Jia NING, Stephanie CLARK,

期刊论文

Cross-sectional network analysis of plasma proteins/metabolites correlated with pathogenesis and therapeutic

期刊论文

Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU

期刊论文

Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis

Hui Wan,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhou

期刊论文